low melting point agarose (Thermo Fisher)

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Thermo Fisher low melting point agarose
Low Melting Point Agarose, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/low+melting+point+agarose/Agarose+LM%2C+low+melt%2C+for+recovery+of+samples+after+separation/bio_rxiv__64898__2026__06__16__732437-48-23-25
Average 95 stars, based on 1 article reviews

low melting point agarose - by Bioz Stars, 2026-09

95/100 stars

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Article Title: Defensin‐DNA Supramolecular Complexes as a New pH‐Sensing Nanomaterial
Article Snippet: At 2 dpf, Tg(kdrl:EGFP) embryos were mounted in 0.8% low melting point agarose (Thermo Fisher Scientific) supplemented with 0.08 mg/mL tricaine.

Article Title: Adhesion-driven rigidity transition decoupled from density-driven jamming triggers epithelial organization in embryonic tissues
Article Snippet: Embryos and explants were mounted in 0.6% low-melting-point agarose (Invitrogen, catalog number 16,520-050) in Viventis Deep sample holders (Single Well Pocket 850, 20533021, and Single Well Pocket 1000, 20533022), and four-dimensional datasets were acquired as z stacks with a step size of 2–4 μm at 15- or 20-min intervals.

Article Title: Reduction of PALS1/Nok disrupts retinal lamination through altered cell positioning while preserving photoreceptor self-organization capacity
Article Snippet: Embryos at 36 hpf were dechorionated, anesthetized in 0.04 mg/mL tricaine methanesulfonate (MS-222; Sigma-Aldrich) diluted in E3 medium, and mounted laterally in 1% low-melting-point agarose (Invitrogen, 16520) supplemented with 0.04 mg/mL MS-222 and 0.003% PTU to prevent melanization, in glass-bottom dishes (MatTek).

Article Title: Distinct evolutionary trajectories of two integration centres, the central complex and mushroom bodies, across Heliconiini butterflies
Article Snippet: Chemical compound, drug , Low melting-point Agarose , Thermo Fisher Scientific, MA, USA , Cat. #: 16520–050 , See Materials and methods for details.

Microscopy:

Article Title: Rhythmic protein condensation in the circadian regulation of glycogen metabolism
Article Snippet: Briefly, the S. elongatus culture, grown and entrained as described above, was removed from the plate, and 1 μL drops were spotted on a 6-well glass-bottom plate (MatTek Corporation). .. Each 1 μL spot was covered with a small square pad (∼5 mm x 5 mm x 1 mm) made of 2% Low Melting Point Agarose (ThermoFisher Scientific) dissolved in BG11. (LMPA + BG11) The plate was then transferred to a epifluorescence microscope (IX71, Olympus Inc.) with a motorized stage (Prior Scientific Inc.). μManager (Edelstein et al., 2010) was used to mark fields of view containing cells to be observed for experiments, and experiments were controlled by a custom μManager script. .. Cells were imaged with a 100x NA=1.3 oil-immersion objective (Olympus) and an Andor Luca EMCCD camera (Oxford Instruments).

Single Cell Gel Electrophoresis:

Article Title: Fuzuloparib enhances radiosensitivity of gain-of-function mutant p53 R273H NSCLC by promoting SLC7A11/GSH/GPX4 axis mediated ferroptosis.
Article Snippet: Total intracellular GSH from cells (six-well plate) was assayed using a Glutathione kit (Beyotime, Shanghai, China) according to the manufacturer's instructions. .. Comet assay H1299 cells (10 × 103) were treated with IR (2 Gy) or FZ + IR 2h before harvesting, resuspended in 0.6% low melting point agarose (UltraPure LMP Agarose (Life Technologies)), placed onto precoated slides with 1% normal melting point agarose, and coverslipped. ..

Staining:

Article Title: Senescent cells induce vascular MHC II to recruit CD4 + T cells and drive inflammation in aging adipose tissue
Article Snippet: Following these washes, samples were incubated in secondary antibodies or dye diluted in blocking solution for 5-7 days in 4°C (anti-rabbit IgG Alexa Fluor 568, 1: 500, Abcam; anti-mouse IgG Alexa Fluor 488, 1: 500, Thermo Fisher Scientific; Hydrazide iFluor 790, 1:500, ATT Bioquest; Isolectin GS-IB4 Alexa Fluor 647, 5 μg/mL, Thermo Fisher Scientific). .. After washes, stained tissues were embedded in 1% low melting point agarose (Invitrogen) and dehydrated through a graded series of 25%, 50%, 75%, and three 100% (v/v) methanol/ H 2 O, with each step lasting 1 hour. ..

Sterility:

Article Title: AI-Accelerated Two-Photon FLIM Phasor Imaging for Label-Free Mapping of Macrophages in Influenza A Virus-Infected Human Lung Tissue
Article Snippet: .. Lung lobes were filled with a mixture of phenol-red-free DMEM/F12 medium (Thermo Fisher Scientific) and 4% low-melting-point agarose (Thermo Fisher Scientific) under sterile conditions. .. Subsequently, precision-cut lung slices (PCLS) of 300 μm thickness were prepared from the agarose-filled lung lobes using a vibratome (Leica VT1200S, Leica, Illinois, USA).

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